0172250 - UEB-Q 20023030 RIV RU eng J - Journal Article
Piruzian, S. - Goldenkova, V. - Lenets, A. - Cvikrová, Milena - Macháčková, Ivana - Kobets, N. - Mett, V.
Physiological and biochemical characteristics of tobacco transgenic plants expressing bacterial dioxygenase.
Russian Journal of Plant Physiology. Roč. 49, č. 6 (2002), s. 817-822. ISSN 1021-4437. E-ISSN 1608-3407
R&D Projects: GA MŠMT LN00A081; GA ČR GA206/00/1354
Institutional research plan: CEZ:AV0Z5038910
Keywords : phenylalanine ammonia lyase * polyphenol oxidases * biosynthesis
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.102, year: 2002

Expression of the 1,2-dihydroxynaphthalene dioxygenase (nahC) gene from Pseudomonas putida in tobacco transgenic plants produces notable phenotypic and biochemical changes: retarded growth and rooting and earlier flowering; chlorotic and necrotic spots on leaves; and a threefold increase in the total phenolics in the leaves of 6-week-old plants (94.51 mug/g fr wt as compared to 33.18 mug/g fr wt in the control) and in the phenylalanine-ammonia lyase activity in 4- week-old plants (0.035 U/g fr wt as compared to 0.014 U/g in the control plants of the same age). The transgenic plants expressing the nahC bacterial gene may serve as a model to study the putative functions of dioxygenases and phenol compounds in plant growth, development, and stress responses.
Permanent Link: http://hdl.handle.net/11104/0069289