LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera)

0135716 - ENTU-I 20010139 RIV CZ eng A - Abstract
Šimek, Petr - Lepša, L. - Kodrík, Dalibor
LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera).
Collection Symposium Series. Roč. 4, - (2001), s. 90-92
[Biologically Active Peptides Conference /7./. 25.04.2001-27.04.2001, Praha]
R&D Projects: GA ČR GA203/01/1586; GA MŠMT ME 417
Institutional research plan: CEZ:AV0Z5007907
Subject RIV: CE - Biochemistry

An LC/ESI/MS/MS method was developed for quantitative analysis of peptide from the adipokinetic hormone/red pigment concentrating hormone family (AKH/RPCH family) in insects. With the developed methodology low femtomole levels can be detected allowing thus direct AKH sequencing and analysis in the corpora cardiaca (CC) from one individual insect Using this method the second AKH in the CC of P. apterus was identified:pGlu-Leu-Thr-Phe-Thr-Pro- Asn-Trp-NH2: the peptide was called the Pya-AKH-II
Permanent Link: http://hdl.handle.net/11104/0033496