0104637 - BFU-R 20043019 RIV GB eng J - Journal Article
Hyršl, P. - Lojek, Antonín - Číž, Milan - Kubala, Lukáš
Chemiluminescence of lucigenin is dependent on experimental conditions.
[Chemiluminiscence lucigeninu je závislá na experimentálních podmínkách.]
Luminescence. Roč. 19, č. 2 (2004), s. 61-63. ISSN 1522-7235. E-ISSN 1522-7243
R&D Projects: GA ČR GA524/02/0395
Institutional research plan: CEZ:AV0Z5004920
Keywords : chemiluminescence * lucigenin * light irradiation
Subject RIV: BO - Biophysics
Impact factor: 1.297, year: 2004

The aim of the study was to test the effect of experimental conditions such as light radiation and temperature on chemiluminescence (CL) of 10-2-10-5 mol/L lucigenin dissolved in various types of solvents. Irradiation by UV light (280, 297, 313 or 400 nm) induced a significant increase in CL of lucigenin dissolved in borate buffer. This effect was the most obvious for 10-2-10-3 mol/L lucigenin. All wavelengths used had a similar effect. UV irradiation did not induce changes in the CL activity of lucigenin dissolved in dH2O or in dimethyl sulphoxide (DMSO). Different results for various solvents were not dependent on pH. The CL activity of 10-2 mol/L and 10-3 mol/L lucigenin dissolved in borate buffer increased depending on the solution temperature (25°C, 30°C, 37°C or 40°C) already at the beginning of the analysis, with a further increase during 16 h incubation period.

Cílem studie bylo testovat vliv experimentálních podmínek jako světelná radiace a teplota na chemiluminiscenci (CL) 10-2-10-5 mol/L lucigeninu rozpuštěného v různých typech rozpouštědel. Ozáření UV světlem (280, 297, 313 nebo 400 nm) indukovalo významné zvýšení CL 10-2-10-3 mol/L lucigeninu rozpuštěného v borátovém pufru. CL aktivita lucigeninu se zvyšovala také v závislosti na teplotě roztoku (25°C, 30°C, 37°C or 40°C). Všechny použité vlnové délky vykazovaly podobný efekt.
Permanent Link: http://hdl.handle.net/11104/0011903