Membrane domain of cyanobacterial ferrochelatase with chlorophyll binding motif is involved in regulation of ferrochelatase activity and is required for its proper function

0027807 - MBÚ 2006 RIV SIGLE PL eng C - Conference Paper (international conference)
Sobotka, Roman - Zuberová, M. - Tichý, Martin
Membrane domain of cyanobacterial ferrochelatase with chlorophyll binding motif is involved in regulation of ferrochelatase activity and is required for its proper function.
[Membranova domena sinicove ferrochelatazy, majici motiv pro vazbu chlorofylu, se ucastni regulace ferrochelatazove aktivity a je nutna pro radnou funkci tohoto enzymu.]
European Workshop on the Molecular Biology of cyanobacteria /6./. Graňsk, 2005, s. 109-109.
[European Workshop on the Molecular Biology of cyanobacteria /6./. Gdaňsk (PL), 25.09.2005-28.09.2005]
Institutional research plan: CEZ:AV0Z50200510
Keywords : chlorophyll * photosystem II
Subject RIV: EE - Microbiology, Virology

To query significance of Cab domain for ferochelatase regulation Synechocystis strains with mutated putative chlorophyll ligands in the Cab domain were prepared. Whereas these mutations did not influence ferochalatase activity in wild type background, same mutations in mutants lacking photosystem I or II resulted in decrease of FeCH activity nearly to the wild type level

Abychom ověřili možnou regulaci aktivity ferochelatázy vazbou chlorofylu na Cab doménu, připravili jsme kmeny, kde byla předpokládaná místa pro vazbu chlorofylu v Cab doméně mutována. Tyto mutace neovlivnily aktivitu ferochalatázy ve standardním kmeni, ale výrazně snížily aktivitu v kmenech, které postrádají fotosytém I nebo II
Permanent Link: http://hdl.handle.net/11104/0117852