Počet záznamů: 1

Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants

  1. 1.
    0370255 - BC-A 2012 RIV CZ eng J - Článek v odborném periodiku
    Pavingerová, Daniela - Bříza, Jindřich - Niedermeierová, Hana - Vlasák, Josef
    Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants.
    Journal of Forest Science. Roč. 57, č. 7 (2011), s. 277-280 ISSN 1212-4834
    Grant CEP: GA MZe QH71290
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: Agrobacterium tumefaciens * genetic engineering * GUS activity * Picea abies (L.) Karst
    Kód oboru RIV: EB - Genetika a molekulární biologie

    In conifers and other plants with long reproductive cycles, transformed embryogenic calli can serve as a convenient source of plant material for the testing insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic callus was obtained after transformation of somatic embryos of Norway spruce (Picea abies) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only with suitable embryogenic line sensitive to Agrobacterium. From nine embryogenic lines tested only one gave transgenic callus
    Trvalý link: http://hdl.handle.net/11104/0204106