Počet záznamů: 1

Sample drift correction in 3D fluorescence photoactivation localization microscopy

  1. 1.
    0370168 - FGU-C 2012 RIV US eng J - Článek v odborném periodiku
    Mlodzianoski, M. J. - Schreiner, J. M. - Callahan, S. P. - Smolková, Katarína - Dlasková, Andrea - Šantorová, Jitka - Ježek, Petr - Bewersdorf, J.
    Sample drift correction in 3D fluorescence photoactivation localization microscopy.
    Optics Express. Roč. 19, č. 16 (2011), s. 15009-15019 ISSN 1094-4087
    Grant CEP: GA ČR GAP302/10/0346; GA MŠk(CZ) ME09029; GA AV ČR(CZ) KJB500110902
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: Biplane FPALM microscopy * mitochondria * sample drift correction
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 3.587, rok: 2011

    The recent development of diffraction-unlimited far-field fluorescence microscopy has overcome the classical resolution limit of similar to 250 nm of conventional light microscopy by about a factor of ten. The improved resolution, however, is susceptible to sample drift on a much finer scale than previously relevant. Here we discuss an approach that corrects for three-dimensional (3D) drift in images of fixed samples without the requirement for fiduciary markers or instrument modification. By imaging mitochondria with Biplane FPALM we show our algorithm’s feasibility in a practical application
    Trvalý link: http://hdl.handle.net/11104/0204047