Počet záznamů: 1

In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain

  1. 1.
    0370061 - UMG-J 2012 RIV US eng J - Článek v odborném periodiku
    Jing, Y. - Machoň, Ondřej - Hampl, Aleš - Dvořák, P. - Xing, Y. - Krauss, S.
    In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain.
    Cellular and Molecular Neurobiology. Roč. 31, č. 5 (2011), s. 715-727 ISSN 0272-4340
    Grant CEP: GA ČR GA204/08/1618
    Výzkumný záměr: CEZ:AV0Z50520514; CEZ:AV0Z50390512
    Klíčová slova: embryonic stem cells * differentiation * neocortex
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.969, rok: 2011

    Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including neocortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons. Several differentiation protocols were used. GFP-positive neocorical cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 12-day incubation. In contrast, protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFP-positive cells. As shown by immunohistochemistry and qRT-PCR, neocortical cells generated from qEBs expressed characteristic genes such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. Activation of Wnt signaling further stimulated generation of D6-GFP-positive cells. qEBs culture also allowed the formation of pseudo-polarized neuroepithelium with the apical N-cadherin and basal Pax6 that is typical of developing neocortex.
    Trvalý link: http://hdl.handle.net/11104/0203973