Počet záznamů: 1

Evaluation of basic mitochondrial functions using rat tissue homogenates

  1. 1.
    0369964 - FGU-C 2012 RIV NL eng J - Článek v odborném periodiku
    Pecinová, Alena - Drahota, Zdeněk - Nůsková, Hana - Pecina, Petr - Houštěk, Josef
    Evaluation of basic mitochondrial functions using rat tissue homogenates.
    Mitochondrion. Roč. 11, č. 5 (2011), s. 722-728 ISSN 1567-7249
    Grant CEP: GA MZd(CZ) NS9759; GA ČR(CZ) GAP303/11/0970; GA MŠk(CZ) 1M0520; GA MŠk OC08017
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: oxidative phosphorylation * isolated mitochondria * tissue homogenates * respiratory control * membrane potential
    Kód oboru RIV: FB - Endokrinologie, diabetologie, metabolizmus, výživa
    Impakt faktor: 3.615, rok: 2011

    The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential and BN/SDS-PAGE Western blotting demonstrated that tissue homogenates can fully substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50–100 mg of the tissue are required for the complex OXPHOS analysis
    Trvalý link: http://hdl.handle.net/11104/0203900