Počet záznamů: 1

Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall

  1. 1.
    0369816 - UOCHB-X 2012 RIV US eng J - Článek v odborném periodiku
    Vinterová, Zuzana - Šanda, Miloslav - Dostál, Jiří - Hrušková-Heidingsfeldová, Olga - Pichová, Iva
    Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall.
    Protein Science. Roč. 20, č. 12 (2011), s. 2004-2012 ISSN 0961-8368
    Grant CEP: GA MŠk(CZ) LC531; GA ČR GA310/09/1945
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: Candida parapsilosis * secreted aspartic proteinases * Sapp1p * cell wall * biotin * proteolytic activity
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 2.798, rok: 2011

    We found that Sapp1p of C. parapsilosis displays enzyme activity prior to secretion, and therefore, it is probably fully folded within the upper layer of the cell wall. The positioning of cell surface-associated Sapp1p was detected by cell wall protein labeling using biotinylation agents, extraction of cell wall proteins by beta-mercaptoethanol, immunochemical detection, and mass spectrometry analysis. The retaining of Sapp1p in the cell wall is not a totally random process and pathogenic yeasts might use this cell-associated proteinase activity to enhance degradation of appropriate substrates.
    Trvalý link: http://hdl.handle.net/11104/0203794