Počet záznamů: 1

A non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite in Bcr-Abl positive cells

  1. 1.
    0368600 - UEB-Q 2012 RIV NL eng J - Článek v odborném periodiku
    Mlejnek, P. - Novák, Ondřej - Doležel, P.
    A non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite in Bcr-Abl positive cells.
    Talanta. Roč. 83, č. 5 (2011), s. 1466-1471 ISSN 0039-9140
    Grant CEP: GA ČR GA301/08/1649
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: K562 cells * P-glycoprotein * Multidrug resistance * N-desmethyl imatinib * CGP 74588
    Kód oboru RIV: EF - Botanika
    Impakt faktor: 3.794, rok: 2011

    Multidrug resistance (MDR) is often associated with overexpression of the P-glycoprotein (P-gp, ABCB1). It was demonstrated that the P-gp mediated efflux decreases the drug concentration in cancer cells which results in the failure of chemotherapy. However, the MDR phenotype in cancer cells obviously involves various mechanisms. Therefore, if we want to estimate a contribution of the P-gp expression to the MDR phenotype, a clear quantitative relationship between the intracellular drug level and cell sensitivity must be established. To achieve this goal, a sensitive and non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite N-desmethyl imatinib (CGP 74588) has been developed.
    Trvalý link: http://hdl.handle.net/11104/0202899