Počet záznamů: 1

Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin

  1. 1.
    0368520 - UEB-Q 2012 RIV NL eng J - Článek v odborném periodiku
    Klemš, M. - Slámová, Z. - Motyka, Václav - Malbeck, Jiří - Trávníčková, Alena - Macháčková, Ivana - Holík, Josef - Procházka, S.
    Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin.
    Plant Growth Regulation. Roč. 65, č. 3 (2011), s. 427-437 ISSN 0167-6903
    Grant CEP: GA MŠk 1M06030; GA ČR(CZ) GAP506/11/0774; GA AV ČR IAA600380701
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Beta-glucosidase * Cytokinin oxidase/ dehydrogenase * Dihydrozeatin
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.604, rok: 2011

    The uptake and metabolism of trans-zeatin (Z) and/or dihydrozeatin (DHZ), in correlation with cytokinin oxidase/dehydrogenase (CKX) and glucosidase activity, were studied in leaf segments derived from wild-type (WT) and transgenic (T) tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) during in vitro induction of shoot organogenesis. T explants harbored the maize gene Zm-p60.1-glucosidase. Higher levels of shoot regeneration were observed on T explants in the early stages of cultivation. In WT explants, the content of cytokinin (CK)-O- and N-glucosides increased. In T explants, a higher content of Z-9-riboside and its monophosphate and higher CKX activity during the early stage of cultures were found. A positive correlation was obtained for bioactive CK content and the organogenic response in T explants. Our results indicate a connection between the organogenic capacity of tobacco explants, metabolism of endogenous CKs and uptake of exogenous CKs from the cultivation medium.
    Trvalý link: http://hdl.handle.net/11104/0202845