Počet záznamů: 1

Activation of Cumulus Cell SMAD2/3 and Epidermal Growth Factor Receptor Pathways Are Involved in Porcine Oocyte-Cumulus Cell Expansion and Steroidogenesis

  1. 1.
    0368348 - UZFG-Y 2012 RIV US eng J - Článek v odborném periodiku
    Nagyová, Eva - Camaioni, A. - Scsuková, S. - Mlynarčíková, A. - Procházka, Radek - Němcová, Lucie - Salustri, A.
    Activation of Cumulus Cell SMAD2/3 and Epidermal Growth Factor Receptor Pathways Are Involved in Porcine Oocyte-Cumulus Cell Expansion and Steroidogenesis.
    Molecular Reproduction and Development. Roč. 78, č. 6 (2011), s. 391-402 ISSN 1040-452X
    Grant CEP: GA ČR GA523/08/0111
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: MURAL GRANULOSA-CELLS * IN-VITRO MATURATION * PREOVULATORY OVARIAN-FOLLICLES
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.532, rok: 2011

    Several lines of evidence suggest that in mice the activation of SMAD2/3 signaling by oocyte secreted factors, together with epidermal growth factor receptor (EGFR) activation, is essential to induce cumulus expansion. Here we show that inhibition of EGFR kinase in follicle stimulating hormone (FSH)-stimulated porcine oocyte-cumulus cell complex (OCCs) strongly decreases hyaluronan (HA) synthesis and its retention in the matrix, as well as progesterone synthesis. Although porcine cumulus cells undergo expansion independently of oocytes, we use biochemical and gene expression analyses to show that they do require activation of SMAD2/3 for optimal stimulation of HA synthesis and proteins involved in the organization of this polymer in the expanded matrix. Furthermore, FSH-induced progesterone synthesis by porcine cumulus cells was increased by blocking SMAD2/3 activation.
    Trvalý link: http://hdl.handle.net/11104/0202719