Počet záznamů: 1

Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo

  1. 1.
    0368327 - UZFG-Y 2012 RIV JP eng J - Článek v odborném periodiku
    Vacková, I. - Nováková, Z. - Krylov, V. - Okada, K. - Kott, T. - Fulka, H. - Motlík, Jan
    Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo.
    Journal of Reproduction and Development. Roč. 57, č. 5 (2011), s. 594-603 ISSN 0916-8818
    Grant CEP: GA MZe QI101A166; GA MŠk 1M0538
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: Blastocyst * In vivo and in vitro * Pig * Stem cells
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.459, rok: 2011

    The present study was designed to extensively characterize cell lines derived from porcine blastocysts by several methodical approaches, including morphological observation, cytogenetic analysis, estimation of alkaline phosphatase activity and detection of specific marker expression at the mRNA/protein level. A comparison was made between the properties of cell lines isolated from in vivo- and in vitro-obtained blastocysts. Our results showed that 57.1% of the in vivo-obtained blastocysts attached to the feeder layer and that 33.3% of them started to grow in a monolayer. The percentage of attached in vitro-produced blastocysts was lower (24.6%), and only 6.9% of them started to grow. Outgrowths from the in vitro-produced blastocysts formed mainly trophectoderm or epithelial-like monolayer, whereas the in vivo-obtained blastocysts formed heterogeneous outgrowths that also contained cells with embryonic stem (ES)-like morphology.
    Trvalý link: http://hdl.handle.net/11104/0202704
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