Počet záznamů: 1

Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix

  1. 1.
    0368158 - UEM-P 2012 RIV US eng J - Článek v odborném periodiku
    Prosecká, Eva - Rampichová, Michala - Vojtová, L. - Tvrdík, D. - Melčáková, Š. - Juhásová, Jana - Plencner, Martin - Jakubová, Radka - Jančář, J. - Nečas, A. - Kochová, P. - Klepáček, J. - Tonar, Z. - Amler, Evžen
    Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix.
    Journal of Biomedical Materials Research. Part A. 99A, č. 2 (2011), s. 307-315 ISSN 1549-3296
    Grant CEP: GA MŠk 2B06130; GA ČR GAP304/10/1307; GA AV ČR IAA500390702; GA MŠk 7E09088
    Grant ostatní: GA ČR.(CZ) GA202/09/1151; GA ČR(CZ) GP106/09/P226; MŠk(CZ) GAUK119009; MŠk(CZ) GAUK96610; MŠk(CZ) GAUK119209; MŠk(CZ) GAUK97110; GA MŠk(CZ) 1M0510
    Výzkumný záměr: CEZ:AV0Z50390512; CEZ:AV0Z50390703; CEZ:AV0Z50450515
    Klíčová slova: collagen/HA scaffold * MSCs * osteoblasts
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 2.625, rok: 2011

    Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ε-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with a thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Cle arly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm.
    Trvalý link: http://hdl.handle.net/11104/0202585