Počet záznamů: 1

Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment

  1. 1.
    0367748 - UZFG-Y 2012 RIV CZ eng J - Článek v odborném periodiku
    Juhásová, Jana - Juhás, Štefan - Klíma, Jiří - Strnádel, Ján - Holubová, Monika - Motlík, Jan
    Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment.
    Physiological Research. Roč. 60, č. 3 (2011), s. 559-571 ISSN 0862-8408
    Grant CEP: GA MŠk 1M0538; GA MŠk 2B06130
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: miniature pig * mesenchymal stem cells * cell differentiation
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.555, rok: 2011

    Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation.
    Trvalý link: http://hdl.handle.net/11104/0202308
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