Počet záznamů: 1

Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy

  1. 1.
    0367418 - BC-A 2012 RIV US eng J - Článek v odborném periodiku
    Vancová, Marie - Šlouf, Miroslav - Langhans, Jan - Pavlová, Eva - Nebesářová, Jana
    Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy.
    Microscopy and Microanalysis. Roč. 17, č. 5 (2011), s. 810-816 ISSN 1431-9276
    Grant CEP: GA AV ČR KAN200520704; GA AV ČR KJB600960906; GA ČR GAP205/10/0348
    Výzkumný záměr: CEZ:AV0Z60220518; CEZ:AV0Z40500505
    Klíčová slova: electron microscopy * colloidal palladium * nanoparticles * labeling * salivary glands * Ixodes ricinus
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 3.007, rok: 2011

    The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans.
    Trvalý link: http://hdl.handle.net/11104/0202116