Počet záznamů: 1

Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants

  1. 1.
    0367361 - BC-A 2012 RIV IT eng J - Článek v odborném periodiku
    Petrzik, Karel - Sarkisova, Tatiana - Čurnová, L.
    Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants.
    Bulletin of Insectology. Roč. 64, Suppl. (2011), s. 25-26 ISSN 1721-8861
    Grant CEP: GA MZe QH91224
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: Plasmid * universal primers * rep gene
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 0.592, rok: 2011

    Rep gene occurring in the majority of plasmids associated with phytoplasmas was selected as a target for real-time PCR together with single-copy Tuf gene from the phytoplasma genome. Rep-specific primers amplified products from host plants infected with phytoplasmas of groups 16SrI, -II, -III, -V, -X, and -XII. Relative concentrations of plasmids ranged depending on strain from 1-2 copies to several hundred copies per each phytoplasma studied. Primers targeting the rep gene are proposed for direct PCR detection of phytoplasma plasmids from experimental periwinkle host as well as from natural plant hosts.
    Trvalý link: http://hdl.handle.net/11104/0202070