Počet záznamů: 1

Next Generation Sequencing-Based Analysis of Repetitive DNA in the Model Dioceous Plant Silene latifolia

  1. 1.
    0367358 - BC-A 2012 RIV US eng J - Článek v odborném periodiku
    Macas, Jiří - Kejnovský, Eduard - Neumann, Pavel - Novák, Petr - Koblížková, Andrea - Vyskot, Boris
    Next Generation Sequencing-Based Analysis of Repetitive DNA in the Model Dioceous Plant Silene latifolia.
    PLoS ONE. Roč. 6, č. 11 (2011), e27335 E-ISSN 1932-6203
    Grant CEP: GA MŠk(CZ) OC10037; GA MŠk(CZ) LC06004; GA MŠk(CZ) LH11058; GA ČR(CZ) GAP501/10/0102; GA ČR(CZ) GAP305/10/0930
    Výzkumný záměr: CEZ:AV0Z50510513; CEZ:AV0Z50040702
    Klíčová slova: Plant genome * Sequencing-Based Analyses * Repetitive DNA * Silene latifolia
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.092, rok: 2011

    We performed low-pass 454 sequencing followed by similarity-based clustering of 454 reads in order to identify and characterize sequences of all major groups of S. latifolia repeats. Illumina sequencing data from male and female genomes were also generated and employed to quantify the genomic proportions of individual repeat families. The majority of identified repeats belonged to LTR-retrotransposons, constituting about 50% of genomic DNA, with Ty3/gypsy elements being more frequent than Ty1/copia. While there were differences between the male and female genome in the abundance of several repeat families, their overall repeat composition was highly similar. Specific localization patterns on sex chromosomes were found for several satellite repeats using in situ hybridization with probes based on k-mer frequency analysis of Illumina sequencing data.
    Trvalý link: http://hdl.handle.net/11104/0202067