Počet záznamů: 1

Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping

  1. 1.
    0367249 - BC-A 2012 RIV NL eng J - Článek v odborném periodiku
    Patzak, J. - Matoušek, Jaroslav
    Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping.
    Biologia Plantarum. Roč. 55, č. 4 (2011), s. 761-765 ISSN 0006-3134
    Grant CEP: GA ČR GA521/08/0740; GA MZe QH81052
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: cluster analysis * dendrogram * Humulus lupulus * simple sequence repeat
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.974, rok: 2011

    The use of expressed sequence tag-simple sequence repeat (EST-SSR) markers might reflect the better relationship among species or cultivars than markers previously used. The first set of 30 EST-SSR was developed in hop (Humulus lupulus L.). They represent 25 gene loci with total of 1268 EST sequences. They were used for characterization of 11 hop samples and cross-amplification in Humulus japonicus Sieb. et Zucc. The number of alleles per locus ranged from two to nine. The observed and expected heterozygosities ranged from 0.182 to 0.956 and from 0.233 to 0.775, respectively. We used EST-SSR markers for cluster analysis of hop genotypes. Dendrogram well matched with genealogical and geographical data for hop genotypes.
    Trvalý link: http://hdl.handle.net/11104/0201993