Počet záznamů: 1

Complex modulation of peptidolytic activity of cathepsin D by sphingolipids

  1. 1.
    0366668 - UOCHB-X 2012 RIV NL eng J - Článek v odborném periodiku
    Žebrakovská, Iva - Máša, Martin - Srp, Jaroslav - Horn, Martin - Vávrová, K. - Mareš, Michael
    Complex modulation of peptidolytic activity of cathepsin D by sphingolipids.
    Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. Roč. 1811, č. 12 (2011), s. 1097-1104 ISSN 1388-1981
    Grant CEP: GA AV ČR IAA400550705
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: sphingolipid * phospholipid * inhibition * activation * cathepsin D * enzyme regulation
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 5.269, rok: 2011

    Cathepsin D is an aspartic peptidase involved in cellular processes including proliferation and apoptosis. We describe a complex pattern of modulation of the peptidolytic activity of cathepsin D by sphingolipids. A panel of sphingolipid derivatives was screened in a FRET-based assay; these molecules demonstrated negative or positive modulation of cathepsin D peptidolytic activity, depending on the sphingolipid structure. Certain sphingosines and ceramides inhibited cathepsin D, and structural requirements for this inhibitory effect were evaluated. In contrast, monoester phosphosphingolipids, especially ceramide-1-phosphate, were identified as activators of cathepsin D peptidolytic activity Thus, sphingolipids and phosphosphingolipids, known to be antagonistic in their cell-signaling functions, displayed opposite modulation of cathepsin D.
    Trvalý link: http://hdl.handle.net/11104/0201567