Počet záznamů: 1

Narrow-bore core–shell particles and monolithic columns in the analysis of silybin diastereoisomers

  1. 1.
    0366150 - MBU-M 2012 RIV DE eng J - Článek v odborném periodiku
    Marhol, Petr - Gažák, Radek - Bednář, P. - Křen, Vladimír
    Narrow-bore core–shell particles and monolithic columns in the analysis of silybin diastereoisomers.
    Journal of Separation Science. Roč. 34, 16-17 (2011), s. 2206-2213 ISSN 1615-9306
    Grant CEP: GA MŠk(CZ) ME10027; GA ČR GAP207/10/0288
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: chromolith * kinetex * Core-shell
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 2.733, rok: 2011

    Two chromatographic narrow-bore columns, a novel 2.6 µm particle-packed KinetexTM C18 core–shell (50x2.1mm id) and monolithic Chromolith® FastGradient RP-18e (50x2mm id), were evaluated for the analysis of diastereoisomers of the flavonolignans silybin and 23-O-acetylsilybin under isocratic conditions. The main advantages of the core–shell column are markedly higher efficiency (hmin=2.8 versus 5.6 for silybin A) and better peak symmetry. The Kinetex column exhibits only a slight change in the height equivalent of the theoretical plate with a higher linear velocity of the mobile phase. The monolithic column shows notably higher selectivity in terms of selectivity factor (1.21 versus 1.12) in the analysis of critical-pair of diastereoisomers (silybin A and silybin B) and enables shorter run duration (approx. twofold) together with lower backpressure
    Trvalý link: http://hdl.handle.net/11104/0201225