Počet záznamů: 1
Clonal analysis of hematopoietic progenitor cells in the zebrafish
0365457 - UMG-J 2012 RIV US eng J - Článek v odborném periodiku
Stachura, D.L. - Svoboda, Ondřej - Lau, R.P. - Balla, K.M. - Zon, L.I. - Bartůněk, Petr - Traver, D.
Clonal analysis of hematopoietic progenitor cells in the zebrafish.
Blood. Roč. 118, č. 5 (2011), s. 1274-1282 ISSN 0006-4971
Grant CEP: GA ČR GA310/08/0878; GA ČR GAP305/10/0953
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: zebrafish * in vitro culture * hematopoiesis
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 9.898, rok: 2011
Identification of hematopoietic progenitor cells in the zebrafish has been hindered by a lack of functional assays to gauge proliferative potential and differentiation capacity. To investigate the nature of myeloerythroid progenitor cells, we developed clonal methylcellulose assays utilizing recombinant zebrafish erythropoietin (Epo) and granulocyte colony stimulating factor (Gcsf). Epo was required to support erythroid colony formation, and Gcsf to support the formation of myeloid colonies. Colonies showed distinct morphologies and were easily scored by their expression of lineage-specific fluorescent transgenes. The majority of progenitor activity was contained within the precursor light scatter fraction, and more immature precursors were present within the lymphoid fraction. These studies provide the first report of clonal hematopoietic progenitor assays in the zebrafish, and establish the number, characteristics, and kinetics of myeloerythroid progenitors during hematopoiesis.
Trvalý link: http://hdl.handle.net/11104/0200694
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