Počet záznamů: 1

Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells

  1. 1.
    0365189 - UMG-J 2012 RIV FR eng J - Článek v odborném periodiku
    Szabo, Pavol - Kolář, Michal - Dvořánková, B. - Lacina, L. - Štork, J. - Vlček, Čestmír - Strnad, Hynek - Tvrdek, M. - Smetana, K.
    Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells.
    Biology of the Cell. Roč. 103, č. 5 (2011), s. 233-248 ISSN 0248-4900
    Grant CEP: GA MŠk 2B06106; GA MŠk(CZ) 1M0520
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: basal cell carcinoma (BCC) * stem cell * tumour stroma
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.600, rok: 2011

    Multipotent mesenchymal stem cells can participate in the formation of a microenvironment stimulating the aggressive behaviour of cancer cells. Cells exhibiting pluripotent embryonic stem cell markers (Nanog and Oct4) have been observed in many tumours. Here, we investigate the stem cell supporting role of cancer-associated fibroblasts (CAF). We test the influence of CAF isolated from basal cell carcinoma on mouse 3T3 fibroblasts, focusing on the expression of stem cell markers and plasticity in vitro. We demonstrate the biological activity of CAF by influencing the 3T3 fibroblasts to express markers such as Oct4, Nanog and Sox2 and to show differentiation potential similar to mesenchymal stem cells. The role of growth factors such as IGF2, FGF7, LEP, NGF and TGFβ, produced by CAF, is established to participate in their activity. Our observations indicate the pro-stem cell activity of CAF and underline the role of epithelial–mesenchymal interaction in tumour biology.
    Trvalý link: http://hdl.handle.net/11104/0200489
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