Počet záznamů: 1

Frequency of aneuploidy related to age in porcine oocytes

  1. 1.
    0365035 - UZFG-Y 2012 RIV US eng J - Článek v odborném periodiku
    Horňák, M. - Jeseta, M. - Musilová, P. - Pavlok, Antonín - Kubelka, Michal - Motlík, Jan - Rubeš, J. - Anger, Martin
    Frequency of aneuploidy related to age in porcine oocytes.
    PLoS ONE. Roč. 6, č. 4 (2011), s. 1-5 E-ISSN 1932-6203
    Grant CEP: GA ČR GA523/09/0743; GA AV ČR IAA501620801
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: porcine * oocytes * aneuploidy
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 4.092, rok: 2011

    It is generally accepted that mammalian oocytes are frequently suffering from chromosome segregation errors during meiosis I, which have severe consequences, including pregnancy loss, developmental disorders and mental retardation. In a search for physiologically more relevant model than rodent oocytes to study this phenomenon, we have employed comparative genomic hybridization (CGH), combined with whole genome amplification (WGA), to study the frequency of aneuploidy in porcine oocytes, including rare cells obtained from aged animals. Using this method, we were able to analyze segregation pattern of each individual chromosome during meiosis I. In contrast to the previous reports where conventional methods, such as chromosome spreads or FISH, were used to estimate frequency of aneuploidy, our results presented here show, that the frequency of this phenomenon was overestimated in porcine oocytes.
    Trvalý link: http://hdl.handle.net/11104/0200375