Počet záznamů: 1

Experimental Examination of EFL and MATX Eukaryotic Horizontal Gene Transfers: Coexistence of Mutually Exclusive Transcripts Predates Functional Rescue

  1. 1.
    0364810 - BC-A 2012 RIV US eng J - Článek v odborném periodiku
    Szabová, J. - Růžička, Petr - Verner, Zdeněk - Hampl, V. - Lukeš, Julius
    Experimental Examination of EFL and MATX Eukaryotic Horizontal Gene Transfers: Coexistence of Mutually Exclusive Transcripts Predates Functional Rescue.
    Molecular Biology and Evolution. Roč. 28, č. 8 (2011), s. 2371-2378 ISSN 0737-4038
    Grant CEP: GA ČR GA204/09/1667; GA MŠk 2B06129
    Grant ostatní: GA ČR(CZ) GAP506/11/1320
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: EFL * MATX * horizontal gene transfer * functional rescue * RNAi * Trypanosoma
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 5.550, rok: 2011

    Elongation factor 1 alpha (EF-1 alpha) and methionine adenosyl transferase (MAT) are enzymes with complicated evolutionary histories. These essential enzymes occur as two relatively divergent paralogs (EF-1 alpha/EFL, MAT/MATX) that have patchy distributions in eukaryotic lineages. To understand the evolution of these paralogs, we have performed in vivo experiments in Trypanosoma brucei – we have demonstrated that EF-1 alpha and MAT can be simultaneously expressed with EFL and MATX, respectively, without affecting the growth of the flagellates. After the endogenous MAT or EF-1 alpha was downregulated by RNA interference, MATX immediately substituted for its paralog, whereas EFL was not able to substitute for EF-1 alpha, leading to mortality. We conclude that MATX is naturally capable of evolving patchy paralog distribution via HGTs and/or long- term coexpression and differential losses. The capability of EFL to spread by HGT is lower.
    Trvalý link: http://hdl.handle.net/11104/0200192