Počet záznamů: 1

Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster

  1. 1.
    0364369 - BC-A 2012 RIV US eng J - Článek v odborném periodiku
    Košťál, Vladimír - Korbelová, J. - Rozsypal, J. - Zahradníčková, Helena - Cimlová, Jana - Tomčala, Aleš - Šimek, Petr
    Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster.
    PLoS ONE. Roč. 6, č. 9 (2011), e25025 E-ISSN 1932-6203
    Grant CEP: GA ČR GA206/07/0269; GA ČR GA203/09/2014
    Výzkumný záměr: CEZ:AV0Z50070508
    Klíčová slova: Drosophila melanogaster
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 4.092, rok: 2011

    We found that long-term cold acclimation considerably improved cold tolerance in fully grown third-instar larvae of D. melanogaster. A comparison of the larvae acclimated at constant 25°C with those acclimated at constant 15°C followed by constant 6°C for 2 d (15°C→6°C) showed that long-term cold acclimation extended the lethal time for 50% of the population (Lt50) during exposure to constant 0°C as much as 630-fold (from 0.137 h to 86.658 h). Such marked physiological plasticity in Lt50 (in contrast to LLT) suggested that chronic indirect chilling injury at 0°C differs from that caused by cold shock. Long-term cold acclimation modified the metabolomic profiles of the larvae. Accumulations of proline (up to 17.7 mM) and trehalose (up to 36.5 mM) were the two most prominent responses. In addition, restructuring of the glycerophospholipid composition of biological membranes was observed.
    Trvalý link: http://hdl.handle.net/11104/0199868