Počet záznamů: 1

Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C-terminus of Potato virus X coat protein in bacterial and plant cells

  1. 1.
    0364095 - UEB-Q 2012 RIV US eng J - Článek v odborném periodiku
    Plchová, Helena - Moravec, Tomáš - Hoffmeisterová, Hana - Folwarczna, Jitka - Čeřovská, Noemi
    Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C-terminus of Potato virus X coat protein in bacterial and plant cells.
    Protein Expression and Purification. Roč. 77, č. 2 (2011), s. 146-152 ISSN 1046-5928
    Grant CEP: GA ČR GA521/09/1525
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Bacterial expression * Human papillomavirus * Oncoprotein E7
    Kód oboru RIV: EI - Biotechnologie a bionika
    Impakt faktor: 1.587, rok: 2011

    The E7 oncoprotein from Human papillomavirus type 16 (HPV16) is an attractive candidate for anti-cancer therapeutical vaccine development. In this study, we engineered different fusions of mutagenized coding sequence of E7 oncoprotein (E7ggg) with coat protein of Potato virus X (PVX CP) both on 5`- and 3`-terminus of PVX CP and evaluated the influence of the length of linker (no linker, 4, 15 aa) connecting PVX CP and E7ggg on their production. At first the expression in Escherichia coil was conducted to assess the characteristics of the recombinant protein prior to be further produced in plants, that is, resultant proteins were used for screening of their immunological reactivity with antibodies against PVX CP and E7. Fusion proteins successfully expressed in bacteria and plants were partially purified and their reactivity and ability to form virus-like particles were evaluated with anti-E7 antibodies.
    Trvalý link: http://hdl.handle.net/11104/0199666
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