Time-resolved selected ion flow tube mass spectrometric quantification of the volatile compounds generated by E. coli JM109 cultured in two different media

0363279 - ÚFCH JH 2012 RIV GB eng J - Článek v odborném periodiku
Chippendale, T. W. E. - Španěl, Patrik - Smith, D.
Time-resolved selected ion flow tube mass spectrometric quantification of the volatile compounds generated by E. coli JM109 cultured in two different media.
Rapid Communications in Mass Spectrometry. Roč. 25, č. 15 (2011), s. 2163-2172. ISSN 0951-4198. E-ISSN 1097-0231
Grant CEP: GA ČR GA203/09/0256
Výzkumný záměr: CEZ:AV0Z40400503
Klíčová slova: SIFT-MS * breath analysis * in-vitro
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 2.790, rok: 2011

Preliminary measurements have been made of the volatile compounds emitted by the bacterium E. coli JM109 cultured in the commonly used media Dulbecco's modified Eagle's medium (DMEM) and lysogeny broth (LB) using selected ion flow tube mass spectrometry, SIFT-MS, as a step towards the real time, non-invasive monitoring of accidental infections of mammalian cell cultures. In one procedure, the culture medium alone and the E. coli cells/medium combination were held at 37 degrees C in bottles sealed with septa for a given time period, usually overnight, to allow the bacterium to proliferate, after which the captured headspace was analysed directly by SIFT-MS. Several compounds were seen to be produced by the E. coli cells that depended on the liquid medium used: when cultured in DMEM, copious amounts of ethanol, acetaldehyde and hydrogen sulphide were produced; in LB ammonia is the major volatile product.
Trvalý link: http://hdl.handle.net/11104/0006530