Počet záznamů: 1

The use of oligoperoxide-coated magnetic nanoparticles to label stem cells

  1. 1.
    0363241 - UMCH-V 2012 RIV US eng J - Článek v odborném periodiku
    Šponarová, Daniela - Horák, Daniel - Trchová, Miroslava - Jendelová, Pavla - Herynek, V. - Mitina, N. - Zaichenko, A. - Stoika, R. - Lesný, Petr - Syková, Eva
    The use of oligoperoxide-coated magnetic nanoparticles to label stem cells.
    Journal of Biomedical Nanotechnology. Roč. 7, č. 3 (2011), s. 384-394 ISSN 1550-7033
    Grant CEP: GA ČR GA203/09/1242; GA ČR GAP503/10/0664; GA MŠk 1M0538; GA AV ČR KAN201110651; GA AV ČR(CZ) KAN401220801
    Výzkumný záměr: CEZ:AV0Z40500505; CEZ:AV0Z50390703
    Klíčová slova: magnetic * nanoparticles * stem cells
    Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
    Impakt faktor: 4.216, rok: 2011

    Iron oxide nanoparticles obtained by the coprecipitation of Fe(II) and Fe(III) salts and oxidation were coated with a novel poly(vinyl acetate-co-5-tert-(butylperoxy)-5-methylhex-1-en-3-yne-co-butyl acrylate-co-maleic anhydride) (PVBM) oligomer to ensure colloidal stability. The magnetic nanoparticles were thoroughly characterized by a range of physico-chemical methods, which proved the presence of the coating on the particles. Experiments with rat mesenchymal stem cells (rMSCs) confirmed that PVBM-coated γ-Fe2O3 nanoparticles were not cytotoxic and that the average efficiency of stem cell labeling was good and comparable to that obtained with commercial agents. The cells labeled with PVBM-coated γ-Fe2O3 nanoparticles displayed excellent contrast on magnetic resonance (MR) images. Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers possibility of additional modification by grafting compounds that reduce non-specific protein adsorption.
    Trvalý link: http://hdl.handle.net/11104/0199248