Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

0362853 - MBÚ 2012 RIV US eng J - Článek v odborném periodiku
Řezanka, Tomáš - Siřišťová, L. - Matoulková, D. - Sigler, Karel
Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium.
Lipids. Roč. 46, č. 8 (2011), 765-780. ISSN 0024-4201. E-ISSN 1558-9307
Grant CEP: GA MŠMT 1M0570
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Plasmalogens * Pectinatus * Liquid chromatography-electrospray tandem mass spectrometry
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.129, rok: 2011

Liquid chromatography–electrospray tandem mass spectrometry (LC/ESI–MS/MS) was used to analyze phospholipids from three species of the anaerobic beerspoilage bacterial genus Pectinatus. Analysis of total lipids by HILIC (Hydrophilic Interaction Liquid Chromatography) column succeeded in separating diacyl- and plasmalogen phospholipids. Plasmalogens were then analyzed by means of the ESI–MS/MS and more than 220 molecular species of four classes of plasmalogens (PlsCho (choline plasmalogen), PlsEtn (ethanolamine plasmalogen), PlsGro (glycerol plasmalogen), and PlsSer (serine plasmalogen)) were identified. Major molecular species were c-p19:0/15:0 PlsEtn and PlsSer, which accounted for more than 4 percent of the total lipids
Trvalý link: http://hdl.handle.net/11104/0199039