Počet záznamů: 1

Fluorescence method for determining the mechanism and speed of action of surface-active drugs on yeast cells

  1. 1.
    0362824 - MBU-M 2012 RIV US eng J - Článek v odborném periodiku
    Kodedová, M. - Sigler, Karel - Lemire, B. D. - Gášková, Dana
    Fluorescence method for determining the mechanism and speed of action of surface-active drugs on yeast cells.
    Biotechniques. Roč. 50, č. 1 (2011), 58-63 ISSN 0736-6205
    Grant CEP: GA MŠk 1M0570
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: fluorescent dye diS-C(3)(3) * membrane potential * surface-active drug
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.669, rok: 2011

    New antifungal agents are needed to treat life-threatening fungal infections, particularly with the development of resistance. Surface-active antifungals have the advantages of minimizing host toxicity and the emergence of drug resistance. We have developed a time-dependent drug exposure assay that allows us to rapidly investigate the mechanism of surface-active antifungal drug action. The assay uses a multidrug pump-deficient strain of Saccharomyces cerevisiae and the potentiometric dye 3,3-dipropylthiacarbocyanine iodide [diS-C3(3)] and can assess whether cells are depolarized, hyperpolarized, or permeabilized by drug exposure
    Trvalý link: http://hdl.handle.net/11104/0199022