Počet záznamů: 1

Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen

  1. 1.
    0362689 - BC-A 2012 RIV US eng J - Článek v odborném periodiku
    Košťál, Vladimír - Zahradníčková, Helena - Šimek, Petr
    Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen.
    Proceedings of the National Academy of Sciences of the United States of America. Roč. 108, č. 32 (2011), s. 13041-13046 ISSN 0027-8424
    Grant CEP: GA ČR GA206/07/0269; GA ČR GA203/09/2014
    Výzkumný záměr: CEZ:AV0Z50070508
    Klíčová slova: insect freeze tolerance * cold hardiness * glass transition
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 9.681, rok: 2011

    The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196°C) in a fully hydrated state. We examined the associations between the physiological and biochemical parameters of differently acclimated larvae and their freeze tolerance. Profiling of 61 different metabolites identified proline as a prominent compound whose concentration increased from 20 to 147 mM during diapause transition and subsequent cold acclimation. This study provides direct evidence for the essential role of proline in high freeze tolerance. Differential scanning calorimetry analysis suggested that high proline levels, in combination with a relatively low content of osmotically active water and freeze dehydration, increased the propensity of the remaining unfrozen water to undergo a glass-like transition (vitrification) and thus facilitated the prevention of cryoinjury.
    Trvalý link: http://hdl.handle.net/11104/0198942