Počet záznamů: 1

Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged 3 antigens: Preparation, structural study and immune response towards rHsp90

  1. 1.
    0361499 - FZU-D 2012 RIV NL eng J - Článek v odborném periodiku
    Mašek, J. - Bartheldyová, E. - Turánek-Knotigová, P. - Škrabalová, M. - Korvasová, Z. - Plocková, J. - Koudelka, Š. - Škodová, P. - Kulich, P. - Křupka, M. - Zachová, K. - Czerneková, L. - Horynová, M. - Kratochvílová, Irena - Miller, A. D. - Zyka, Daniel - Michálek, J. - Vrbová, J. - Šebela, M. - Ledvina, Miroslav - Raška, M. - Turánek, J.
    Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged 3 antigens: Preparation, structural study and immune response towards rHsp90.
    Journal of Controlled Release. Roč. 151, č. 2 (2011), s. 193-201 ISSN 0168-3659
    Grant CEP: GA ČR(CZ) GAP304/10/1951; GA AV ČR KAN200520703; GA AV ČR KAN200100801
    Výzkumný záměr: CEZ:AV0Z10100520; CEZ:AV0Z40550506
    Klíčová slova: recombinant vaccine * liposome
    Kód oboru RIV: EC - Imunologie
    Impakt faktor: 5.732, rok: 2011
    http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3D-520M1P7-1&_user=625012&_coverDate=01%2F21%2F2011&_rdoc=1&_fmt=high&_orig=gateway&_origi

    Hsp90-CA is present in cell wall of Candida pseudohyphae or hyphae - typical pathogenic morphotype for 31 both systemic and mucosal Candida infections. Heat shock protein from Candida albicans (hsp90-CA) is an 32 important target for protective antibodies during disseminated candidiasis of experimental mice and human. 33 His-tagged protein rHsp90 was prepared and used as the antigen for preparation of experimental 34 recombinant liposomal vaccine. Nickel-chelating liposomes (the size around 100 nm, PDI≤0.1) were 35 prepared from the mixture of egg phosphatidyl choline and nickel-chelating lipid DOGS-NTA-Ni (molar ratio 36 95:5%) by hydration of lipid film and extrusion methods. New non-pyrogenic hydrophobised derivative of 37 MDP (C18-O-6-norAbuMDP) was incorporated into liposomes as adjuvans. by metallochelating bond and the structure of these proteoliposomes 39 was studied by dynamic light scattering, AF microscopy, TEM and GPC.
    Trvalý link: http://hdl.handle.net/11104/0198800