Počet záznamů: 1

Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove

  1. 1.
    0360994 - UOCHB-X 2012 RIV US eng J - Článek v odborném periodiku
    Kielkowski, Pavel - Pohl, Radek - Hocek, Michal
    Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove.
    Journal of Organic Chemistry. Roč. 76, č. 9 (2011), s. 3457-3462 ISSN 0022-3263
    Grant CEP: GA MŠk LC512; GA ČR GA203/09/0317
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: nucleoside triphosphates * functionalized DNA * cross-coupling reactions * nucleic-acids
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 4.450, rok: 2011

    Cytosin-5-yl ethynyl derivatives of pyrimidine and 7-deazaadenine 2-deoxyribonucleosides and nucleoside triphosphates (dNTPs) were prepared in one step by the aqueous Sonogashira coupling of unprotected halogenated nucleos(t)ides with 5-ethynylcytosine. The modified dNTPs were good substrates for DNA polymerases suitable for primer extension or PCR construction of DNA bearing acetylene-linked cytosine(s) in the major groove mimicking the flipped-out nucleotide.
    Trvalý link: http://hdl.handle.net/11104/0198417