Počet záznamů: 1

Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids

  1. 1.
    0360819 - UMCH-V 2012 RIV NL eng J - Článek v odborném periodiku
    Tishchenko, Galina - Šimůnek, Jiří - Bartoňová, Hana - Dušková, Jarmila - Dohnálek, Jan - Ponomareva, Evgenia - Tennikova, T.
    Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids.
    Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 879, č. 22 (2011), s. 2175-2178 ISSN 1570-0232
    Grant CEP: GA ČR GA310/09/1407; GA ČR GA525/05/2584
    Výzkumný záměr: CEZ:AV0Z40500505; CEZ:AV0Z50450515
    Klíčová slova: sample preparation * culture fluids C. paraputrificum J4 * chitinases
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.888, rok: 2011

    Membrane ultrafiltration (UF) was used in sample preparation of the culture fluids of the human intestinal bacterium Clostridium paraputrificum strain J4 containing seven extracellular chitinolytic isoenzymes (38–90 kDa). The subsequent filtration of the bacteria-free supernatants was carried out through Millipore membranes with cut-off 100 and 30 kDa for separation of undigested components of the culture medium and bacterial metabolites with molecular weight higher and lower than that of the target enzymes. On the basis of the obtained experimental results, the sample preparation procedure of the culture fluids of C. paraputrificum J4 was established to be used further in chromatographic separations of the chitinolytic enzymes.
    Trvalý link: http://hdl.handle.net/11104/0198281