Počet záznamů: 1

High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRs

  1. 1.
    0360788 - MBU-M 2012 RIV US eng J - Článek v odborném periodiku
    Cantarella, L. - Gallifuoco, A. - Malandra, A. - Martínková, Ludmila - Spera, A. - Cantarella, M.
    High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRs.
    Enzyme and Microbial Technology. Roč. 48, 4-5 (2011), 345-350 ISSN 0141-0229
    Grant CEP: GA MŠk OC09046
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Nitrile hydratase-amidase cascade system * 3-Cyanopyridine bioconversion * Nicotinic acid
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.367, rok: 2011

    High yields of nicotinic acid from 3-cyanopyridine bioconversion were obtained by exploiting the in situ nitrile hydratase–amidase enzymatic cascade system of Microbacterium imperiale CBS 498-74. Experiments were carried out in continuously stirred tank UF-membrane bioreactors (CSMRs) arranged in series. This reactor configuration enables both enzymes, involved in the cascade reaction, to work with optimized kinetics, without any purification, exploiting their differing temperature dependences. To this end, the first CSMR, optimized for the properties of the NHase, was operated (i) at low temperature (5◦C), limiting inactivation of the more fragile enzyme, nitrile hydratase, (ii) with a high residence time (24h) to overcome reaction rate limitation
    Trvalý link: http://hdl.handle.net/11104/0198255