Polymer brushes showing non-fouling in blood plasma challenge the currently accepted design of protein resistant surfaces

0360787 - ÚMCH 2012 RIV DE eng J - Článek v odborném periodiku
Rodriguez-Emmenegger, Cesar Adolfo - Brynda, Eduard - Riedel, Tomáš - Houska, Milan - Šubr, Vladimír - Bologna Alles, A. - Hasan, E. - Gautrot, J. E. - Huck, W. T. S.
Polymer brushes showing non-fouling in blood plasma challenge the currently accepted design of protein resistant surfaces.
Macromolecular Rapid Communications. Roč. 32, č. 13 (2011), s. 952-957. ISSN 1022-1336. E-ISSN 1521-3927
Grant CEP: GA AV ČR KAN200670701; GA AV ČR IAAX00500803
Výzkumný záměr: CEZ:AV0Z40500505
Klíčová slova: biosensors * blood plasma * HPMA
Kód oboru RIV: CD - Makromolekulární chemie
Impakt faktor: 4.596, rok: 2011

Ultra-low-fouling poly[N-(2-hydroxypropyl) methacrylamide] (poly(HPMA)) brushes have been synthesized for the first time. Similar to the so far only ultra-low-fouling surface, poly(carboxybetaine acrylamide), the level of blood plasma fouling was below the detection limit of surface plasmon resonance (SPR, 0.03 ng • cm−2) despite being a hydrogen bond donor and displaying a moderate wettability, thus challenging the currently accepted views for the design of antifouling properties. The antifouling properties were preserved even after two years of storage. To demonstrate the potential of poly(HPMA) brushes for the preparation of bioactive ultra-low fouling surfaces a label-free SPR immunosensor for detection of G Streptococcus was prepared.
Trvalý link: http://hdl.handle.net/11104/0198254