Počet záznamů: 1

A comprehensive study in triblock copolymer membrane interaction

  1. 1.
    0360337 - UFCH-W 2012 RIV NL eng J - Článek v odborném periodiku
    Pembouong, G. - Morellet, N. - Kral, Teresa - Hof, Martin - Scherman, D. - Bureau, M.-F. - Mignet, N.
    A comprehensive study in triblock copolymer membrane interaction.
    Journal of Controlled Release. Roč. 151, č. 1 (2011), s. 57-64 ISSN 0168-3659
    Grant CEP: GA AV ČR IAA400400621; GA MŠk(CZ) LC06063
    Výzkumný záměr: CEZ:AV0Z40400503
    Klíčová slova: poloxamer * pluronic * liposomes
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 5.732, rok: 2011

    Poloxamers are triblock copolymers made of poly(ethylene glycol)-(poly(propylene glycol))-poly(ethylene glycol). They have been shown to enhance gene transfer in the muscle, and co-administration of polymers and DNA appeared to be crucial to obtain this effect. It is questionable then if some interaction occurs between polymers and DNA. Polymer interaction with membranes represents a second crucial point due to the central hydrophobic part of the triblock copolymers. Besides, the question of the polymer spanning or adsorbing to the surface has not been solved by now. We addressed these issues by means of sensitive techniques that allowed working in diluted conditions and gaining in comprehension of gene transfection. By means of simultaneous time-correlated single-photon counting and fluorescence correlation spectroscopy, we have shown that the diffusion time of a single DNA molecule and PicoGreen lifetime was not altered in the presence of the triblock copolymer L64.
    Trvalý link: http://hdl.handle.net/11104/0197913