Počet záznamů: 1

The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria

  1. 1.
    0360066 - BC-A 2012 RIV CZ eng J - Článek v odborném periodiku
    Koubová, Anna - Knapp, B.A. - Insam, H. - Pižl, Václav - Elhottová, Dana
    The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria.
    Acta Societatis Zoologicae Bohemicae. Roč. 74, - (2010), s. 69-74 ISSN 1211-376X.
    [Central European Workshop on Soil Zoology /10./. České Budějovice, 21.04.2009-24.04.2009]
    Grant CEP: GA MŠk LC06066; GA MŠk MEB060814; GA AV ČR IAA600200704
    Výzkumný záměr: CEZ:AV0Z60660521
    Klíčová slova: soil zoology * soil microbiology * microbial diversity
    Kód oboru RIV: EH - Ekologie - společenstva

    The changes that occurred in the diversity of microbes in soil and compost during its passage through the gut of earthworms were evaluated in two epigeic earthworm species Eisenia fetida (Savigny, 1826) and E. andrei (Bouché, 1972), which inhabit forest soil and garden organic compost, respectively. A modified extraction protocol was used to extract DNA from forest soil, compost and fresh earthworm faeces. DNA was amplified in a polymerase chain reaction (PCR). The products were analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprinting. Analysis of archaeal and bacterial DGGE fingerprints revealed that the diversity in both microbial domains increased after passage through the guts of E. andrei, but not E. fetida. Nevertheless, cluster and correspondence analyses revealed that the passage of soil and compost through the guts of E. fetida, E. andrei, respectively, had a significant effect on the composition of the archaea.
    Trvalý link: http://hdl.handle.net/11104/0197705