Počet záznamů: 1

Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma

  1. 1.
    0359480 - UZFG-Y 2012 RIV US eng M - Část monografie knihy
    Sheng, S. - Skalníková, Helena - Meng, A. - Fu, Q. - Everett, A. - Van Eyk, J.
    Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma.
    Serum/Plasma Proteomics Metods and Protocols. NEW YORK: Humana Press, 2011, s. 29-46. ISBN 978-1-61779-067-6
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: liquid chromatography * chromatofocusing * reversed phase
    Kód oboru RIV: EB - Genetika a molekulární biologie

    Serum and plasma-based biomarker discovery requires technologies with specific capabilities: sufficient proteome coverage and depth; technical reproducibly; and the scalability to enable analysis on substantial volumes of a large number of samples at reasonable cost. We have shown that plasma samples processed using ProteomeLab™ IgY-12 LC10 Proteome Partitioning Kits to selectively remove the 12 most highly-abundant proteins, followed by intact protein separation by two-dimensional liquid chromatography (2DLC, chromatofocusing and reversed phase) can unique enrich for middle –lower-abundant proteins. Equally, 1DLC (reversed phase) separation of intact proteins is complementary to 2DLC. The serial use of a single piece of equipment can be prohibitively time-consuming and thus, this chapter also describes the harmonization of multiple LC instruments in order to minimize technical variation and ensure reproducibility.
    Trvalý link: http://hdl.handle.net/11104/0197263