Počet záznamů: 1

Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry

  1. 1.
    0359308 - UEB-Q 2011 RIV NL eng J - Článek v odborném periodiku
    Tarkowski, Petr - Václavíková, Kateřina - Novák, Ondřej - Pertry, I. - Hanuš, Jan - Whenham, R. - Vereecke, D. - Šebela, M. - Strnad, Miroslav
    Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry.
    Analytica Chimica Acta. Roč. 680, 1-2 (2010), s. 86-91 ISSN 0003-2670
    Grant CEP: GA ČR GA301/08/1649
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Cytokinins * Rhodococcus fascians * High-performance liquid chromatography
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 4.310, rok: 2010

    A sensitive and reliable high-performance liquid chromatographic method with tandem mass spectrometric detection has been developed and used for the determination of 2-methylthio-cytokinin derivatives produced by the phytopathogenic actinomycete Rhodococcus fascians. The cultivation medium containing secreted cytokinins was concentrated and subjected to a solid-phase extraction (C18 and ion-exchange). The purified samples were further separated and analyzed by HPLC-ESI-MS/MS. This allowed to achieve chromatographic resolution of six highly hydrophobic cytokinin species including 2-methylthio-isopentenyladenine, 2-methylthio-isopentenyladenosine, 2-methylthio-trans-zeatin and 2-methylthio-trans-zeatin riboside and their cis-isomers. Quantification was performed by a standard isotope dilution method using a multiple-reaction monitoring (MRM) mode.
    Trvalý link: http://hdl.handle.net/11104/0197112