Počet záznamů: 1

PCR-RFLP assays to distinguish the Western and Eastern phylogroups in wild and cultured tench Tinca tinca

  1. 1.
    0359234 - UZFG-Y 2012 RIV GB eng J - Článek v odborném periodiku
    Lajbner, Zdeněk - Kotlík, Petr
    PCR-RFLP assays to distinguish the Western and Eastern phylogroups in wild and cultured tench Tinca tinca.
    Molecular Ecology Resources. Roč. 11, č. 2 (2011), s. 374-377 ISSN 1755-098X
    Grant CEP: GA MŠk LC06073
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: EPIC * exon-primed intron-crossing marker * mtDNA
    Kód oboru RIV: EG - Zoologie
    Impakt faktor: 3.062, rok: 2011

    The tench Tinca tinca is a valued table fish which is being transplanted between watersheds without concern for genetic similarity to wild populations, and efficient phylogeographic markers were therefore urgently needed to rapidly distinguish genetically distinct geographic populations and to assess their contribution to the hatchery breeds and to the stocked wild populations. The new method developed in this study relies on PCR-RFLP assays of two independent nuclear-encoded markers and of one mitochondrial DNA marker, and allows the rapid identification of the Western and Eastern phylogroup and also of three geographical mtDNA clades within the Eastern phylogroup. The method will be useful for monitoring the introduction and human-mediated spread of the phylogroups in wild populations, for characterisation of cultured strains and in breeding experiments.
    Trvalý link: http://hdl.handle.net/11104/0197058