Počet záznamů: 1

Preparation of stable Pd nanoparticles with tunable size for multiple immunolabeling in biomedicine

  1. 1.
    0359122 - UMCH-V 2012 RIV NL eng J - Článek v odborném periodiku
    Šlouf, Miroslav - Pavlova, Ewa - Bhardwaj, Monika - Pleštil, Josef - Onderková, H. - Philimonenko, Anatoly - Hozák, Pavel
    Preparation of stable Pd nanoparticles with tunable size for multiple immunolabeling in biomedicine.
    Materials Letters. Roč. 65, č. 8 (2011), s. 1197-1200 ISSN 0167-577X
    Grant CEP: GA AV ČR KAN200520704; GA ČR GAP205/10/0348
    Výzkumný záměr: CEZ:AV0Z40500505; CEZ:AV0Z50520514
    Klíčová slova: palladium nanoparticles * nanocrystalline materials * immunolabeling
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.307, rok: 2011

    Stable Pd nanoparticles (PdNP) with a tunable size (3-15 nm) were synthesized by controlled chemical reduction of PdCl2 with sodium citrate in water. The morphology of PdNP was characterized by transmission electron microscopy, while their stability in solution was verified by quasi-elastic light scattering and small-angle X-ray scattering. Intensive stirring of reacting mixture played a vital role in achieving reproducible particle sizes. Controlled changes of pH and initial concentrations were employed in fine-tuning particle size distributions. Finally, 10 nm PdNP were conjugated with goat anti-mouse IgG antibody as proved by electrophoresis (SOS-PAGE) and used for ultrastructural immunolabeling, which confirmed suitability of PdNP for multiple immunolabeling in biomedicine.
    Trvalý link: http://hdl.handle.net/11104/0006427