Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS

0358473 - ÚFCH JH 2012 RIV DE eng J - Článek v odborném periodiku
Macháň, Radek - Hof, Martin - Chernovets, Tatsiana - Zhmak, M. N. - Ovchinnikova, T. V. - Sýkora, Jan
Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS.
Analytical and Bioanalytical Chemistry. Roč. 399, č. 10 (2011), s. 3547-3554. ISSN 1618-2642. E-ISSN 1618-2650
Grant CEP: GA ČR GAP208/10/0376; GA ČR GAP208/10/1090; GA MŠMT(CZ) LC06063
Výzkumný záměr: CEZ:AV0Z40400503
Klíčová slova: arenicin-1 * supported lipid bilayers * antibacterial peptide
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 3.778, rok: 2011

Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are used to detect changes in lipid and peptide diffusion upon addition of unlabelled arenicin-1 to SLBs. Arenicin-1 decreases lipid mobility in negatively charged SLBs. According to diffusion law analysis, microdomains of significantly lower lipid mobility are formed. The analysis of peptide FCS data confirms the presence of microdomains for anionic SLBs. No indications of microdomain formation are detected in SLBs composed purely of zwitterionic lipids. Additionally, our FCS results imply that arenicin-1 exists in the form of oligomers and/or aggregates when interacting with membranes of both compositions.
Trvalý link: http://hdl.handle.net/11104/0196507