Počet záznamů: 1

Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families

  1. 1.
    0358401 - BC-A 2012 RIV NL eng J - Článek v odborném periodiku
    Novotná, Jana - Havelka, Jan - Starý, Petr - Koutecký, P. - Vítková, Magda
    Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families.
    Genetica. Roč. 139, č. 3 (2011), s. 281-289 ISSN 0016-6707
    Grant CEP: GA ČR GA522/09/1940
    Grant ostatní: AV ČR(CZ) KJB501410901; Grant Agency of the University of South Bohemia(CZ) 137/2010/P
    Výzkumný záměr: CEZ:AV0Z50070508
    Klíčová slova: Diuraphis noxia * karyotype * genome size
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.148, rok: 2011

    We investigated physical genomic features in Russsian wheat aphid (Diuraphis noxia), a worldwide pest, by karyotype analysis using differential staining with AgNO3, CMA3, and DAPI, by chromosomal localization of ribosomal DNA (rDNA), H3 and H4 histone genes, and the telomeric sequence (TTAGG)n using fluorescence in situ hybridization (FISH), and by measuring the RWA genome size using flow cytometry. The female karyotype, 2n = 10, has a pair of X chromosomes, whereas the male karyotype, 2n = 9, has a single X. The X chromosome is the largest element in the karyotype and 18S rRNA. H3 and H4 gene clusters are colocalized at one of its ends. Interestingly, D. noxia lacks the (TTAGG)n telomeric sequence in its genome. The female genome size is 2C = 0.86 pg, whereas the male genome size is 2C = 0.70 pg. The X chromosome occupies about 35% of the female haploid genome (1C = 0.43 pg), which makes it one of the largest sex chromosomes in the animal kingdom.
    Trvalý link: http://hdl.handle.net/11104/0196461