Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53

0358220 - ÚFE 2011 RIV DE eng J - Článek v odborném periodiku
Šípová, Hana - Špringer, Tomáš - Homola, Jiří
Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53.
Analytical and Bioanalytical Chemistry. Roč. 399, č. 7 (2011), s. 2343-2350. ISSN 1618-2642. E-ISSN 1618-2650
Grant CEP: GA AV ČR KAN200670701; GA MŠMT OC09058; GA ČR GA202/09/0193
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: Surface Plasmon Resonance * point mutation * amplification
Kód oboru RIV: JB - Senzory, čidla, měření a regulace
Impakt faktor: 3.778, rok: 2011

This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance (SPR) biosensor technology. In this assay, a part of the target DNA is recognized by a probe coupled with streptavidin-oligonucleotide (SON) complexes ex-situ. The SON-target complex is then detected with the SPR sensor with high specificity and sensitivity. The assay is applied to detection of short analogue of TP53 at levels down to 40 pM.
Trvalý link: http://hdl.handle.net/11104/0196317