Počet záznamů: 1

Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53

  1. 1.
    0358220 - URE-Y 2011 RIV DE eng J - Článek v odborném periodiku
    Šípová, Hana - Špringer, Tomáš - Homola, Jiří
    Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53.
    Analytical and Bioanalytical Chemistry. Roč. 399, č. 7 (2011), s. 2343-2350 ISSN 1618-2642
    Grant CEP: GA AV ČR KAN200670701; GA MŠk OC09058; GA ČR GA202/09/0193
    Výzkumný záměr: CEZ:AV0Z20670512
    Klíčová slova: Surface Plasmon Resonance * point mutation * amplification
    Kód oboru RIV: JB - Senzory, čidla, měření a regulace
    Impakt faktor: 3.778, rok: 2011

    This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance (SPR) biosensor technology. In this assay, a part of the target DNA is recognized by a probe coupled with streptavidin-oligonucleotide (SON) complexes ex-situ. The SON-target complex is then detected with the SPR sensor with high specificity and sensitivity. The assay is applied to detection of short analogue of TP53 at levels down to 40 pM.
    Trvalý link: http://hdl.handle.net/11104/0196317