Počet záznamů: 1
Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures
0357035 - UEB-Q 2011 RIV GB eng J - Článek v odborném periodiku
Vondráková, Zuzana - Cvikrová, Milena - Eliášová, Kateřina - Martincová, Olga - Vágner, Martin
Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures.
Tree Physiology. Roč. 30, č. 10 (2010), s. 1335-1348 ISSN 0829-318X
Grant CEP: GA MŠk OC 158; GA MŠk OC08013
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: cryopreservation * Norway spruce (Picea abies (L ) Karst) * somatic embryogenesis
Kód oboru RIV: EA - Morfologické obory a cytologie
Impakt faktor: 2.403, rok: 2010
The anatomical characteristics and PA content during cryopreservation and regrowth were studied in the ESMs of AFO 541 and C110 cultures, which have comparable ESM anatomy but diverse growth rates, PA content and regeneration abilities after cryopreservation. The endogenous free PAs were maintained at high levels, with spermidine being the predominant PA in the AFO 541, while in C110 the content of putrescine and spermidine was almost identical and rather low, the content of spermidine being approximately one-third that in the ESM of AFO 541. Osmotic pretreatment, using a double application of sorbitol followed by an application of dimethyl sulfoxide (DMSO) resulted in the continual disintegration of polyembryogenic centers and suspensors in both cell lines. A continual decrease in the level of PAs was observed during the cell osmotic pretreatment. The cells that retained their viability and regrowth ability after cryopreservation were the meristematic cells inside embryonal heads.
Trvalý link: http://hdl.handle.net/11104/0195407
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