Počet záznamů: 1

Differential expression of non-coding RNAs and continuous evolution of the X chromosome in testicular transcriptome of two mouse species

  1. 1.
    0356977 - UMG-J 2011 RIV US eng J - Článek v odborném periodiku
    Homolka, David - Ivánek, Robert - Forejt, Jiří - Jansa, Petr
    Differential expression of non-coding RNAs and continuous evolution of the X chromosome in testicular transcriptome of two mouse species.
    PLoS ONE. Roč. 6, č. 6 (2011), e17198-e17198 E-ISSN 1932-6203
    Grant CEP: GA ČR GA301/07/1383; GA MŠk(CZ) 1M0520
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: spermatogenesis * tiling array * expression profiles
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.092, rok: 2011

    Tight regulation of testicular gene expression is a prerequisite for male reproductive success, while differentiation of gene activity in spermatogenesis is important during speciation. We compared testicular transcriptomes of two closely related mouse species, Mus musculus and Mus spretus. We analyzed testicular expression using tiling arrays overlapping Chromosomes 2, X, Y and mitochondrial genome. An excess of differentially regulated non-coding RNAs was found on Chromosome 2 including the intronic antisense RNAs, intergenic RNAs and premature piRNAs. This indicates the role of non-coding RNAs in species-specific regulation of spermatogenesis. A striking difference was found in the expression of X-linked G6pdx gene, the parental gene of the autosomal retrogene G6pd2. The postmeiotic expression of G6pdx in Mus spretus points towards the continuous evolution of X-chromosome silencing and provides an example of expression change accompanying the out-of-the X-chromosomal retroposition.
    Trvalý link: http://hdl.handle.net/11104/0195356
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