Počet záznamů: 1

Facile end-labeling of RNA with electroactive Os(VI) complexes

  1. 1.
    0356812 - BFU-R 2011 RIV US eng J - Článek v odborném periodiku
    Trefulka, Mojmír - Bartošík, Martin - Paleček, Emil
    Facile end-labeling of RNA with electroactive Os(VI) complexes.
    Electrochemistry Communications. Roč. 12, č. 12 (2010), s. 1760-1763 ISSN 1388-2481
    Grant CEP: GA ČR(CZ) GPP301/10/P548; GA MŠk(CZ) ME09038; GA MŠk(CZ) LC06035
    Grant ostatní: GA AV ČR(CZ) KAN400310651
    Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
    Klíčová slova: RNA end-labeling * voltammetry * osmium complexes
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 4.282, rok: 2010

    Ribose at the 3'-end of oligonucleotides (oligos) selectively modified by Os(VI)2,2'-bipyridine (bipy) produced two CV redox couples at pyrolytic graphite electrode. Using square wave voltammetry (SWV) 22-mer oligos can be detected down to 250 nM. At mercury electrodes the Os(VI)bipy-oligo adducts produced an electrocatalytic peak at ~-1.2 V allowing their determination down to picomolar concentrations.
    Trvalý link: http://hdl.handle.net/11104/0195238