Removable nanocoatings for siRNA polyplexes

0356597 - ÚMCH 2011 RIV US eng J - Článek v odborném periodiku
Kostka, Libor - Koňák, Čestmír - Šubr, Vladimír - Špírková, Milena - Addadi, Y. - Neeman, M. - Lammers, T. - Ulbrich, Karel
Removable nanocoatings for siRNA polyplexes.
Bioconjugate Chemistry. Roč. 22, č. 2 (2011), s. 169-179. ISSN 1043-1802. E-ISSN 1520-4812
Grant CEP: GA AV ČR IAAX00500803; GA AV ČR KAN200200651
Výzkumný záměr: CEZ:AV0Z40500505
Klíčová slova: polyelectrolyte complexes * siRNA * nanocoating
Kód oboru RIV: CD - Makromolekulární chemie
Impakt faktor: 4.930, rok: 2011

Intracellularly removable polymer nanocoatings designed for surface modification of siRNA-based polyplexes (PEC) were developed with the aim to improve properties of PEC in physiological environment. N-(2-hydroxypropyl)methacrylamide (HPMA) based copolymers containing reactive groups bound via biodegradable disulfide spacers to the polymeric backbone were synthesized and used for coating of PEC formed by the self-assembly of siRNA with the polycations polyethylene imine and poly(HPMA)-grafted poly(l-lysine) (GPL). Light scattering analysis showed the attachment of, on average, 28 polymer molecules to the surface of the polyplexes forming a 5-nm-thick hydrophilic stealth coating. Coating of the siRNA/GPL PEC substantially improved their stability in physiological solutions and an initial proof-of-principle for time- and concentration-dependent specific gene silencing was obtained. It was demonstrated that siRNA/GPL complexes hold significant potential for further development.
Trvalý link: http://hdl.handle.net/11104/0195082